ELECTROCHROMATOGRAPHIC SEPARATION OF PROTEINS

被引:21
作者
BASAK, SK
VELAYUDHAN, A
KOHLMANN, K
LADISCH, MR
机构
[1] PURDUE UNIV, RENEWABLE RESOURCES ENGN LAB, W LAFAYETTE, IN 47907 USA
[2] PURDUE UNIV, DEPT AGR ENGN, W LAFAYETTE, IN 47907 USA
关键词
D O I
10.1016/0021-9673(94)01276-K
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We have developed a modified electrochromatography system which minimizes Joule heating at electric field strengths up to 125 V/cm. A non-linear equilibrium model is described which incorporates electrophoretic mobility, hydrodynamic flow velocity, and an electrically induced concentration polarization at the surface of the stationary phase. This model is able to provide useful estimates of protein retention time and velocity in a column packed with Sephadex gel and subjected to an electric field. A correlation of electrophoretic mobility of peptide and proteins with respect to their charge, molecular mass, and asymmetry enables the selection of solute target molecules for electrochromatographic separations. Good separation of protein mixtures have been obtained.
引用
收藏
页码:69 / 76
页数:8
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