DETERMINATION OF BIOACTIVE PEPTIDES USING CAPILLARY ZONE ELECTROPHORESIS MASS-SPECTROMETRY

被引：135

作者：

MOSELEY, MA

DETERDING, LJ

TOMER, KB

JORGENSON, JW

机构：

[1] NIEHS, MOLEC BIOPHYS LAB, POB 12233, RES TRIANGLE PK, NC 27709 USA

[2] UNIV N CAROLINA, DEPT CHEM, CHAPEL HILL, NC 27514 USA

来源：

ANALYTICAL CHEMISTRY | 1991年 / 63卷 / 02期

关键词：

D O I：

10.1021/ac00002a005

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Mixtures of bioactive peptides have been analyzed by capillary zone electrophoresis/mass spectrometry (CZE/MS) using an on-line coaxial continuous-flow fast atom bombardment interface. High separation efficiencies (up to 410 000 theoretical plates) were obtained from low femtomole levels of peptides. The analysis of basic peptides was accomplished by using aminopropyl-silylated CZE columns to minimize zone broadening due to adsorption effects. CZE/MS/MS data were acquired from femtomole levels of peptides in electrophoretic real time.

引用

页码：109 / 114

页数：6

共 51 条

[1] ALLEN MH, 1989, RAPID COMMUN MASS SP, V3, P255
[2] MICRO AND MICROBORE LIQUID-CHROMATOGRAPHY FAST ATOM BOMBARDMENT-MASS SPECTROMETRY (LC-FAB-MS) ANALYSIS OF PEPTIDES AND ANTIBIOTICS
ASHCROFT, AE
[J]. ORGANIC MASS SPECTROMETRY, 1987, 22 (11): : 754 - 757
[3] FAST ATOM BOMBARDMENT OF SOLIDS (FAB) - A NEW ION-SOURCE FOR MASS-SPECTROMETRY
BARBER, M
BORDOLI, RS
SEDGWICK, RD
TYLER, AN
[J]. JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1981, (07) : 325 - 327
[4] CONTRIBUTIONS OF MASS-SPECTROMETRY TO PEPTIDE AND PROTEIN-STRUCTURE
BIEMANN, K
[J]. BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1988, 16 (1-12): : 99 - 111
[5] ION SPRAY INTERFACE FOR COMBINED LIQUID CHROMATOGRAPHY/ATMOSPHERIC PRESSURE IONIZATION MASS-SPECTROMETRY
BRUINS, AP
COVEY, TR
HENION, JD
[J]. ANALYTICAL CHEMISTRY, 1987, 59 (22) : 2642 - 2646
[6] OPTIMIZATION OF CHROMATOGRAPHIC CONDITIONS FOR COMBINED MICROBORE HPLC CONTINUOUS-FLOW FAST-ATOM BOMBARDMENT MASS-SPECTROMETRY
CAPRIOLI, RM
DAGUE, BB
WILSON, K
[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1988, 26 (12) : 640 - 644
[7] MICROBORE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY FOR THE ANALYSIS OF PROTEOLYTIC DIGESTS BY CONTINUOUS-FLOW FAST-ATOM BOMBARDMENT MASS-SPECTROMETRY
CAPRIOLI, RM
MOORE, WT
DAGUE, B
MARTIN, M
[J]. JOURNAL OF CHROMATOGRAPHY, 1988, 443 : 355 - 362
[8] MICROBORE HPLC-MASS SPECTROMETRY FOR THE ANALYSIS OF PEPTIDE MIXTURES USING A CONTINUOUS-FLOW INTERFACE
CAPRIOLI, RM
DAGUE, B
FAN, T
MOORE, WT
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 146 (01) : 291 - 299
[9] CONTINUOUS-FLOW SAMPLE PROBE FOR FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY
CAPRIOLI, RM
FAN, T
COTTRELL, JS
[J]. ANALYTICAL CHEMISTRY, 1986, 58 (14) : 2949 - 2954
[10] Chen K H, 1988, Rapid Commun Mass Spectrom, V2, P237, DOI 10.1002/rcm.1290021107

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