DETERMINATION OF BIOACTIVE PEPTIDES USING CAPILLARY ZONE ELECTROPHORESIS MASS-SPECTROMETRY

被引：135

作者：

MOSELEY, MA

DETERDING, LJ

TOMER, KB

JORGENSON, JW

机构：

[1] NIEHS, MOLEC BIOPHYS LAB, POB 12233, RES TRIANGLE PK, NC 27709 USA

[2] UNIV N CAROLINA, DEPT CHEM, CHAPEL HILL, NC 27514 USA

来源：

ANALYTICAL CHEMISTRY | 1991年 / 63卷 / 02期

关键词：

D O I：

10.1021/ac00002a005

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Mixtures of bioactive peptides have been analyzed by capillary zone electrophoresis/mass spectrometry (CZE/MS) using an on-line coaxial continuous-flow fast atom bombardment interface. High separation efficiencies (up to 410 000 theoretical plates) were obtained from low femtomole levels of peptides. The analysis of basic peptides was accomplished by using aminopropyl-silylated CZE columns to minimize zone broadening due to adsorption effects. CZE/MS/MS data were acquired from femtomole levels of peptides in electrophoretic real time.

引用

页码：109 / 114

页数：6

共 51 条

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