A LECTIN FROM THE MUSHROOM PHOLIOTA-AURIVELLA

被引：24

作者：

KAWAGISHI, H ^{[1
]}

ABE, Y ^{[1
]}

NAGATA, T ^{[1
]}

KIMURA, A ^{[1
]}

CHIBA, S ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC AGR,DEPT AGR CHEM,SAPPORO,HOKKAIDO 060,JAPAN

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1991年 / 55卷 / 10期

关键词：

D O I：

10.1080/00021369.1991.10871000

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A lectin was isolated from the fruiting bodies of Pholiota aurivella by preparative PAGE and named PAA (Pholiota aurivella agglutinin). PAA was a polymeric protein of more than several hundred kDa consisting of 18-kDa subunits. The amino acids were sequenced from the N-terminus of the lectin; they were YSVTTPNSVKGGTNQG. PAA agglutinated human erythrocytes regardless of blood type equally and Pronase treatment of erythrocytes increased the sensitivity to agglutination by the lectin. In a hemagglutinating inhibition assay, none of the mono- and oligosaccharides used affected agglutination by the lectin. Among glycoproteins, asialofetuin was the best inhibitor. This is first isolated and characterized lectin from the family Strophariaceae.

引用

页码：2485 / 2489

页数：5

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