SEQUENCE-TAGGED NOTI SITES OF HUMAN CHROMOSOME-21 - SEQUENCE-ANALYSIS AND MAPPING

被引：11

作者：

HATTORI, M

TOYODA, A

ICHIKAWA, H

ITO, T

OHGUSU, H

OISHI, N

KANO, T

KUHARA, S

OHKI, M

SAKAKI, Y

机构：

[1] UNIV TOKYO,INST MED SCI,MOLEC MED LAB,TOKYO 108,JAPAN

[2] UNIV TOKYO,INST MED SCI,CTR HUMAN GENOME,TOKYO 108,JAPAN

[3] KYUSHU UNIV,GENET INFORMAT RES LAB,FUKUOKA 812,JAPAN

[4] SAITAMA CANC CTR,INA,SAITAMA 362,JAPAN

[5] KYUSHU UNIV,GRAD SCH GENET RESOURCES TECHNOL,FUKUOKA 812,JAPAN

来源：

GENOMICS | 1993年 / 17卷 / 01期

关键词：

D O I：

10.1006/geno.1993.1280

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We isolated and analyzed 19 NotI site-containing clones specific for human chromosome 21. The overall process consisted of selective isolation of Not I site-containing clones from flow-sorted chromosome 21 libraries, selection of independent clones by their restriction patterns and nucleotide sequences, and assignment of the clones to chromosome 21. Sequence analysis showed that the regions around the NotI sites had features typical of CpG islands, such as extremely high GC content, high-frequency appearance of CpG dinucleotide sequences, and lack of methylation of these CpGs. PCR conditions for these extremely G+C-rich templates were optimized to establish these NotI sites as sequence-tagged sites. © 1993 Academic Press, Inc.

引用

页码：39 / 44

页数：6

共 29 条

[1] HIGH-LEVELS OF DENOVO METHYLATION AND ALTERED CHROMATIN STRUCTURE AT CPG ISLANDS IN CELL-LINES [J].