MOLECULAR-CLONING OF A BOVINE ORNITHINE DECARBOXYLASE CDNA AND ITS USE IN THE DETECTION OF RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS IN HOLSTEINS

被引：10

作者：

YAO, JB ^{[1
]}

ZADWORNY, D ^{[1
]}

KUHNLEIN, U ^{[1
]}

HAYES, JF ^{[1
]}

机构：

[1] MCGILL UNIV,DEPT ANIM SCI,ST ANNE BELLEVUE,PQ H9X 3V9,CANADA

来源：

GENOME | 1995年 / 38卷 / 02期

关键词：

BOVINE; ORNITHINE DECARBOXYLASE; CLONING; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

D O I：

10.1139/g95-041

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cDNA coding for ornithine decarboxylase (ODC) was isolated from a bovine liver cDNA library. The clone (1758 base pairs) consisted of 5'- and 3'-untranslated regions of 185 and 187 nucleotides, respectively, and an open reading frame of 1383 nucleotides encoding an ODC protein (M(r) 51 342 daltons) of 461 amino acids. Comparison of the nucleotide and the predicted amino acid of the cDNA with other mammalian ODCs showed a very high degree of homology both at the DNA and protein levels. The bovine ODC mRNA was identified by northern blot to be a single species with a molecular size of 2.35 kilobase pairs. Primer extension analysis indicated that the 5'-untranslated region of the bovine ODC mRNA was 312 nucleotides long. Southern blot analysis of bovine genomic DNA revealed restriction fragment length polymorphisms when cleaved with restriction enzymes PstI, MspI, TaqI, and BglI.

引用

页码：325 / 331

页数：7

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