PURIFICATION AND CHARACTERIZATION OF GLYOXALASE-I FROM HANSENULA-MRAKII

被引：14

作者：

INOUE, Y ^{[1
]}

TRAN, LT ^{[1
]}

YOSHIKAWA, K ^{[1
]}

MURATA, K ^{[1
]}

KIMURA, A ^{[1
]}

机构：

[1] KYOTO UNIV,FOOD SCI RES INST,UJI,KYOTO 611,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1991年 / 71卷 / 02期

关键词：

D O I：

10.1016/0922-338X(91)90239-D

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Glyoxalase I was purified from Hansenula mrakii IFO 0895 which was resistant to 25 mM methyglyoxal. The molecular weight of the purified enzyme was calculated to be 38,000 by both gel-filtration of Sephadex G-150 and SDS-PAGE. The enzyme was almost specific to methylglyoxal (K(m) = 0.91 mM). The activity of the enzyme was not inhibited by metal ion chelators such as EDTA, which is a potent inhibitor for glyoxalase Is from other sources.

引用

页码：131 / 133

页数：3

共 17 条

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