PURIFICATION AND CHARACTERIZATION OF METHYLGLYOXAL REDUCTASE FROM HANSENULA-MRAKII

被引：7

作者：

INOUE, Y ^{[1
]}

TRAN, LT ^{[1
]}

YOSHIKAWA, K ^{[1
]}

MURATA, K ^{[1
]}

KIMURA, A ^{[1
]}

机构：

[1] KYOTO UNIV, FOOD SCI RES INST, UJI, KYOTO 611, JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1991年 / 71卷 / 02期

关键词：

D O I：

10.1016/0922-338X(91)90240-H

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Methylglyoxal reductase was purified from Hansenula mrakii IFO 0895 to a homogenous state on polyacrylamide gel electrophoresis. The enzyme consisted of a single polypeptide chain with a molecular weight of 34,000. The enzyme was specific to methylglyoxal (K(m) = 1.92 mM) and NADPH (K(m) = 40.8-mu-M). The activity of the enzyme was inhibited by p-chloromercuribenzoate and HgCl2. NADP also inhibited the activity of the enzyme, and the K(i) value was calculated to be 0.25 mM.

引用

收藏

页码：134 / 136

页数：3

相关论文

共 16 条

[1] GEL-FILTRATION BEHAVIOUR OF PROTEINS RELATED TO THEIR MOLECULAR WEIGHTS OVER A WIDE RANGE [J].

ANDREWS, P .

BIOCHEMICAL JOURNAL, 1965, 96 (03) :595-&

[2]

BOSRON WF, 1972, J BIOL CHEM, V247, P4480

[3]

DAVIDSON WS, 1979, J BIOL CHEM, V254, P3724

[4] INDUCTION OF GLUTATHIONE-PEROXIDASE BY LINOLEIC-ACID HYDROPEROXIDE IN HANSENULA-MRAKII [J].

INOUE, Y ;

ICHIRYU, T ;

YOSHIKAWA, K ;

TRAN, LT ;

MURATA, K ;

KIMURA, A .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1990, 54 (12) :3289-3293

[5] METABOLISM OF 2-OXOALDEHYDE IN MOLD - PURIFICATION AND CHARACTERIZATION OF 2 METHYLGLYOXAL REDUCTASES FROM ASPERGILLUS-NIGER [J].

INOUE, Y ;

RHEE, H ;

WATANABE, K ;

MURATA, K ;

KIMURA, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 171 (1-2) :213-218

[6] METABOLISM OF 2-OXOALDEHYDES IN YEASTS - PURIFICATION AND CHARACTERIZATION OF LACTALDEHYDE DEHYDROGENASE FROM SACCHAROMYCES-CEREVISIAE [J].

INOUE, Y ;

WATANABE, K ;

SHIMOSAKA, M ;

SAIKUSA, T ;

FUKUDA, Y ;

MURATA, K ;

KIMURA, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 153 (02) :243-247

[7] PURIFICATION AND CHARACTERIZATION OF GLYOXALASE-I FROM HANSENULA-MRAKII [J].

INOUE, Y ;

TRAN, LT ;

YOSHIKAWA, K ;

MURATA, K ;

KIMURA, A .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1991, 71 (02) :131-133

[8] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[9]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[10] 2-OXOALDEHYDE METABOLISM IN MICROORGANISMS [J].

MURATA, K ;

INOUE, Y ;

RHEE, HI ;

KIMURA, A .

CANADIAN JOURNAL OF MICROBIOLOGY, 1989, 35 (04) :423-431