IDENTIFICATION OF ALPHA-AMYLASE INHIBITOR AS A MAJOR ALLERGEN OF WHEAT-FLOUR

被引：54

作者：

FRANKEN, J ^{[1
]}

STEPHAN, U ^{[1
]}

MEYER, HE ^{[1
]}

KONIG, W ^{[1
]}

机构：

[1] RUHR UNIV BOCHUM,INST PHYSIOL CHEM,PROT STRUKT LAB,W-4630 BOCHUM,GERMANY

来源：

INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | 1994年 / 104卷 / 02期

关键词：

BAKERS ASTHMA; ALLERGEN; WHEAT FLOUR;

D O I：

10.1159/000236726

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

We isolated a major wheat flour allergen with a molecular weight of 15-kD. The 15-kD allergen was purified from an extract of wheat flour by ammonium sulfate precipitation followed by gel filtration. Using the immunoblot technique, IgE, specifically directed towards the single isolated allergen, was detectable in 50% of the applied sera. We determined the N-terminal amino acid sequence of the allergen and identified it as an alpha-amylase inhibitor protein. Our data emphasize that low molecular weight wheat flour allergens play an important role in the pathogenesis of baker's asthma disease.

引用

页码：171 / 174

页数：4

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