KIN17, A MOUSE NUCLEAR ZINC-FINGER PROTEIN THAT BINDS PREFERENTIALLY TO CURVED DNA

被引:43
作者
MAZIN, A
TIMCHENKO, T
DEMURCIA, JM
SCHREIBER, V
ANGULO, JF
DEMURCIA, G
DEVORET, R
机构
[1] CTR UNIV ORSAY,INST CURIE,F-91405 ORSAY,FRANCE
[2] INST BIOL MOLEC & CELLULAIRE,CNRS,UPR 9003,F-67084 STRASBOURG,FRANCE
[3] CNRS,ENZYMOL LAB,F-91198 GIF SUR YVETTE,FRANCE
关键词
D O I
10.1093/nar/22.20.4335
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Kin17 is a 45 kDa protein encoded by the KIN17 gene located on mouse chromosome 2, band A. The kin17 amino acid sequence predicts two domains, which were shown to be functional: (i) a bipartite nuclear localization signal (NLS) that can drive the protein to the cell nucleus, (ii) a bona fide zinc finger of the C2H2 type. The zinc finger is involved in kin17 binding to double-stranded DNA since a mutant deleted of the zinc finger, kin17 Delta 1, showed reduced binding. Single-stranded DNA was bound poorly by kin17. Interestingly, we found that kin17 protein showed preferential binding to curved DNA from either pBR322 or synthetic oligonucleotides. Binding of kin17 to a non-curved DNA segment increased after we had inserted into it a short curved synthetic oligonucleotide. Kin17 Delta 2, a mutant deleted of 110 amino acids at the C-terminal end, still exhibited preferential binding to curved DNA and so did kin17 Delta 1, suggesting that a domain recognizing curved DNA is located in the protein core.
引用
收藏
页码:4335 / 4341
页数:7
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