IMMOBILIZED MAMMALIAN-CELL CULTIVATION IN HOLLOW FIBER BIOREACTORS

被引：19

作者：

PIRET, JM

COONEY, CL

机构：

[1] UNIV BRITISH COLUMBIA, DEPT CHEM ENGN, VANCOUVER V6T 1W5, BC, CANADA

[2] MIT, DEPT CHEM ENGN, CTR BIOTECHNOL PROC ENGN, CAMBRIDGE, MA 02139 USA

来源：

BIOTECHNOLOGY ADVANCES | 1990年 / 8卷 / 04期

关键词：

MAMMALIAN CELL; HOLLOW FIBER; IMMOBILIZED BIOREACTORS; METABOLITE GRADIENTS; TISSUE CAPILLARY;

D O I：

10.1016/0734-9750(90)91996-T

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cultivation of animal cells for the production of recombinant proteins is an important method for manufacturing complex proteins requiring posttranslational processing. One of the often considered methods for cultivation is by immobilization of the cells in hollow fiber bioreactors (HFBRs). These systems allow the cells to grow to high densities in a shear protected environment; furthermore the product can be accumulated in high concentration in the case of ultrafiltration HFBRs. Operation and scale-up are constrained by nutrient and product transport with oxygen transfer to growing cells being the most critical parameter. Mathematical models describing HFBRs have proved to be useful in quantitating and understanding the constraints and guiding the scale-up of this approach to animal cell cultivation.

引用

页码：763 / 783

页数：21

共 100 条

[41] CULTIVATION OF ANIMAL CELLS AT CONTROLLED DISSOLVED OXYGEN PARTIAL PRESSURE [J].