CLONING AND SEQUENCING OF GLUTAMATE MUTASE COMPONENT-E FROM CLOSTRIDIUM-TETANOMORPHUM

被引：7

作者：

BRECHT, M

KELLERMANN, J

PLUCKTHUN, A

机构：

[1] MAX PLANCK INST BIOCHEM, KLOPFERSPITZ 18A, W-8033 MARTINSRIED, GERMANY

[2] UNIV MUNICH, GENZENTRUM, W-8000 MUNICH 2, GERMANY

来源：

FEBS LETTERS | 1993年 / 319卷 / 1-2期

关键词：

GLUTAMATE MUTASE; COBALAMIN-DEPENDENT ENZYME; VITAMIN-B12;

D O I：

10.1016/0014-5793(93)80042-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nucleotide sequence of the large subunit E of glutamate mutase of Clostridium tetanomorphum was determined. The protein consists of 483 amino acids and is not made in a precursor form, thus excluding the possibility of subunit E being a pyruvoyl enzyme. It shows no homology to any other protein in the database, and while binding coenzyme B-12, a conspicuous B-12 binding motif shared amongst other proteins, is not detectable at the sequence level.

引用

页码：84 / 89

页数：6

共 20 条

[11] CLONING, SEQUENCING, AND EXPRESSION IN ESCHERICHIA-COLI OF THE CLOSTRIDIUM-TETANOMORPHUM GENE ENCODING BETA-METHYLASPARTASE AND CHARACTERIZATION OF THE RECOMBINANT PROTEIN [J].