SEMIBATCH PRODUCTION OF FRUCTO-OLIGOSACCHARIDES FROM SUCROSE BY IMMOBILIZED CELLS OF AUREOBASIDIUM-PULLULANS

被引：55

作者：

YUN, JW ^{[1
]}

JUNG, KH ^{[1
]}

OH, JW ^{[1
]}

LEE, JH ^{[1
]}

机构：

[1] CHEIL SUGAR CO LTD,CTR RES & DEV,552-1 DOKPYUNG RI,KYONGGI DO,SOUTH KOREA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1990年 / 24-5卷

关键词：

Aureobasidium pullulans; effectiveness factor; Fructo-oligosaccharides; immobilized cells; semibatch process;

D O I：

10.1007/BF02920254

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Aureobasidium pullulans cells with fructosyltransferase activity were immobilized in 2% calcium alginate beads, and the production of fructo-oligosaccharides from sucrose was studied in a stirred tank bioreactor. It was found that cells of A. pullulans were entrapped evenly on the alginate matrix of 2.2 mm in diameter, and an effectiveness factor of the beads was determined to be 0.3. By comparison with the system of free cells in batch operation, the total amount of fructo-oligosaccharides produced by immobilized cells was similar although the composition of fructo-oligosaccharides was found to be different. In semibatch operation with immobilized cells, reproducible results up to 60 cycles were obtained at 50 °C and this operation resulted in no loss of activity of immobilized cells. © 1990 Humana Press Inc.

引用

页码：299 / 308

页数：10

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