PREPARATION OF THERAPEUTIC PHOSPHOLIPIDS THROUGH PORCINE PANCREATIC PHOSPHOLIPASE A(2)-MEDIATED ESTERIFICATION AND LIPOZYME-MEDIATED ACIDOLYSIS

被引：30

作者：

HOSOKAWA, M

TAKAHASHI, K

KIKUCHI, Y

HATANO, M

机构：

[1] HOKKAIDO UNIV,FAC FISHERIES,DEPT MARINE BIORESOURCES CHEM,HAKODATE,HOKKAIDO 041,JAPAN

[2] MINIST AGR FORESTRY & FISHERIES,NATL FOOD RES INST,INSTRUMENTAT ENGN LAB,TSUKUBA,IBARAKI 305,JAPAN

来源：

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 1995年 / 72卷 / 11期

关键词：

ACIDOLYSIS; DEFORMABILITY; DECOSAHEXAENOIC ACID; EICOSAPENTAENOIC ACID; ESTERIFICATION; NONAQUEOUS SOLVENTS; PHOSPHOLIPIDS; RED BLOOD CELL; UNSATURATED FATTY ACIDS;

D O I：

10.1007/BF02546201

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Highly unsaturated fatty acid-containing phospholipids (HUFA-PL), which serve to increase the deformability of human red blood cells, were prepared through porcine phospholipase A(2)-mediated esterification of the lysophosphatidylcholone, which was derived from soy phosphatidylcholine (PC), and by Lipozyme-mediated acidolysis. Through these processes, phospholipase A(2), with formamide as a water mimic, enhanced the incorporation of HUFA into position sn-2 of PC and suppressed hydrolysis of the synthesized PL. On the other hand, Lipozyme-mediated acidolysis between position sn-1 of soy PC and HUFA was enhanced by a combination of water and propylene glycol. Simultaneously, the recovered PL products showed decreased hydrolysis of newly synthesized health-beneficial HUFA-PL.

引用

页码：1287 / 1291

页数：5