COINCIDENT SEQUENCE CLONING - A NEW APPROACH TO GENOME ANALYSIS

被引:4
作者
BROOKES, AJ
PORTEOUS, DJ
机构
[1] A. J. Brookes and D. J. Porteous are, the MRC Human Genetics Unit, Western General Hospital, Edinburgh, EH4 2XU, Crewe Road
关键词
D O I
10.1016/0167-7799(92)90166-S
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Many analytical molecular genetic techniques developed over the past decade have evolved primarily to tackle the problem of targeting or isolating sequences of interest in complex mixtures. A new approach to this problem, Coincident Sequence Cloning (CSC), involves integrating a pair of DNA mixtures in such a way as to isolate any shared sequence components.
引用
收藏
页码:40 / 44
页数:5
相关论文
共 13 条
[11]   GENOMIC ANALYSIS .1. INHERITANCE UNITS AND GENETIC SELECTION IN THE RAPID DISCOVERY OF LOCUS LINKED DNA MARKERS [J].
SANDA, AI ;
FORD, JP .
NUCLEIC ACIDS RESEARCH, 1986, 14 (18) :7265-7283
[12]  
WEBER JL, 1989, AM J HUM GENET, V44, P388
[13]   USE OF THE POLYMERASE CHAIN-REACTION FOR THE DIFFERENTIAL CROSS SCREENING OF LIBRARIES CLONED INTO PHAGE-LAMBDA-BASED VECTORS [J].
WONG, P ;
MACDONALD, I ;
TENNISWOOD, M .
GENE, 1989, 85 (01) :59-65