DETECTION OF A STAPHYLOCOCCAL ENDO-BETA-N-ACETYLGLUCOSAMINIDASE USING POLYACRYLAMIDE GELS

被引:15
作者
SUGAI, M
KOMATSUZAWA, H
TOMITA, S
AKIYAMA, T
MIYAKE, Y
SUGINAKA, H
机构
[1] Department of Microbiology, Hiroshima University School of Dentistry, Hiroshima
关键词
ACTIVITY GEL; BACTERIOLYTIC ENZYME; ENDO-BETA-N-ACETYLGLUCOSAMINIDASE; RENATURATION; SDS PAGE;
D O I
10.1016/0167-7012(91)90027-N
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An activity gel method has been developed for the detection of bacteriolytic enzymes. After gel electrophoresis of the enzymes in the presence of SDS, enzyme activity was seen in the gel as a transparent band resulting from bacteriolysis in the gels containing bacterial cells. Staphylococcal endo-beta-N-acetylglucosaminidase, as a model enzyme, was employed to see the analytical advantage of this method relative to the turbidimetric method. This activity gel method detects in the order of 100 pg of staphylococcal endo-beta-N-acetylglucosaminidase. The method is simple to use, very sensitive and applicable to renaturable bacteriolytic enzymes.
引用
收藏
页码:11 / 16
页数:6
相关论文
共 17 条
[11]  
OFARRELL PH, 1975, J BIOL CHEM, V250, P4007
[12]  
Rogers HJ., 1979, MICROBIAL CELL WALLS, P437
[13]   NUCLEASE DETECTION IN SDS-POLYACRYLAMIDE GEL-ELECTROPHORESIS [J].
ROSENTHAL, AL ;
LACKS, SA .
ANALYTICAL BIOCHEMISTRY, 1977, 80 (01) :76-90
[14]   MOLECULAR WEIGHT ESTIMATION OF POLYPEPTIDE CHAINS BY ELECTROPHORESIS IN SDS-POLYACRYLAMIDE GELS [J].
SHAPIRO, AL ;
VINUELA, E ;
MAIZEL, JV .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1967, 28 (05) :815-&
[16]  
SUGAI M, 1989, FEMS MICROBIOL LETT, V61, P267, DOI 10.1016/0378-1097(89)90209-7
[17]  
WARD JB, 1984, MICROBIAL CELL WALL, P159