EFFECT OF (S)-1-[(3-HYDROXY-2-PHOSPHONYL METHOXY) PROPYL] CYTOSINE ON THE REPLICATION AND MORPHOGENESIS OF HERPES-SIMPLEX VIRUS TYPE-1

被引：5

作者：

CHATTERJEE, S ^{[1
]}

BURNS, P ^{[1
]}

WHITLEY, RJ ^{[1
]}

KERN, ER ^{[1
]}

机构：

[1] UNIV ALABAMA, DEPT MICROBIOL, BIRMINGHAM, AL 35294 USA

来源：

ANTIVIRAL RESEARCH | 1992年 / 19卷 / 03期

关键词：

HERPES SIMPLEX TYPE-1; HPMPC; MECHANISM OF ACTION; VIRAL REPLICATION; INHIBITION OF;

D O I：

10.1016/0166-3542(92)90078-J

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Treatment of African green monkey kidney cells with 1 mug/ml of (S)-1-[(3-hydroxy-2-phosphonyl methoxy) propyl] cytosine (HPMPC) inhibited the release of infectious herpes simplex virus type 1 (HSV-1) by more than 90%. Electron microscopic observations of HPMPC-treated monkey kidney cells demonstrated few intracellular or extracellular viral particles. The viral particles seen were without dense cores. In addition, HPMPC blocked cell fusion induced by HSV-1 in monkey kidney cells. Immunoblot analysis showed that HPMPC significantly blocked the expression of HSV-1-specific proteins. Furthermore, HPMPC inhibited the synthesis of viral DNA as determined by in situ hybridization. These results indicate that HPMPC inhibits the replication of HSV by blocking one of the events involved in DNA synthesis.

引用

页码：181 / 192

页数：12

共 26 条

[21]

Mertz GJ, 1990, ANTIVIR AGENTS VIR, P265

[22] HERPESVIRUS GLYCOPROTEIN-SYNTHESIS AND INSERTION INTO PLASMA-MEMBRANES [J].

PEAKE, ML ;

NYSTROM, P ;

PIZER, LI .

JOURNAL OF VIROLOGY, 1982, 42 (02) :678-690

[23] (S)-1-(3-HYDROXY-2-PHOSPHONYLMETHOXYPROPYL)CYTOSINE, A POTENT AND SELECTIVE INHIBITOR OF HUMAN CYTOMEGALO-VIRUS REPLICATION [J].

SNOECK, R ;

SAKUMA, T ;

DECLERCQ, E ;

ROSENBERG, I ;

HOLY, A .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1988, 32 (12) :1839-1844

[24] LATENT HERPES-SIMPLEX VIRUS FROM TRIGEMINAL GANGLIA OF RABBITS WITH RECURRENT EYE INFECTION [J].

STEVENS, JG ;

COOK, ML ;

NESBURN, AB .

NATURE-NEW BIOLOGY, 1972, 235 (59) :216-&

[25] LATENT HERPES SIMPLEX VIRUS IN SPINAL GANGLIA OF MICE [J].

STEVENS, JG ;

COOK, ML .

SCIENCE, 1971, 173 (3999) :843-&

[26]

WARREN KG, 1977, LANCET, V2, P637

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