DETERMINATION OF COLCHICINE IN BIOLOGICAL-FLUIDS BY REVERSE-PHASE HPLC - VARIATION OF COLCHICINE LEVELS IN RATS

被引：18

作者：

FERNANDEZ, P

BERMEJO, AM

TABERNERO, MJ

LOPEZRIVADULLA, M

CRUZ, A

机构：

[1] Department of Legal Medicine, Service of Forensic Toxicology, Faculty of Medicine

来源：

FORENSIC SCIENCE INTERNATIONAL | 1993年 / 59卷 / 01期

关键词：

COLCHICINE; REVERSE-PHASE HPLC; RATS;

D O I：

10.1016/0379-0738(93)90307-V

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

A reverse-phase HPLC method for the determination of colchicine in biological fluids is proposed. Blood, liver and kidney colchicine concentrations were determined in rats at different times following intraperitoneal (i.p.) injection of 10 mg/kg of the drug. Colchicine was extracted from the samples studied using dichloromethane at pH 8. The dry extract was redissolved in the mobile phase and analyzed with simultaneous detection at 254 and 350 nm.

引用

页码：15 / 18

页数：4

共 10 条

[1] FLUOROMETRIC ASSAY OF TUBULIN-COLCHICINE COMPLEX [J].