MODIFIED REACTION CENTERS FROM RHODOBACTER-SPHAEROIDES R26 - EXCHANGE OF MONOMERIC BACTERIOCHLOROPHYLL WITH 13(2)-HYDROXY-BACTERIOCHLOROPHYLL

被引：40

作者：

STRUCK, A

SCHEER, H

机构：

[1] Botanisches Institut der Universität, D-8000 München 19

来源：

FEBS LETTERS | 1990年 / 261卷 / 02期

关键词：

(Rhodobacter sphaeroides); 13[!sup]2[!/sup]-Hydroxy-bacteriochlorophyll a; Bacteriochlorophyll a; Bacteriochlorophyll a'; Chlorophyll; Chromatography; Epimerization; Esterifying alcohol; Oxidation; Photosynthesis; Pigment exchange; Pigment modification; Reaction center;

D O I：

10.1016/0014-5793(90)80598-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubation of photosynthetic reaction centers from Rhodobacter sphaeroides R26 with exogenous 132-OH-bacteriochlorophyll ap or aGG according to Scheer et al. (1987) results in the exchange of endogenous bacteriochlorophyll ap. The exchange amounts to ≤ 50% according to HPLC analysis, corresponding to a complete replacement of the 'monomeric' bacteriochlorophylls, bm and bl, by exogenous pigment. The absorption spectra show small, but distinct changes in the Qx-region of the bacteriochlorophylls, and bleaching of the modified reaction centers is retained. The corresponding binding sites must be accessible from the exterior, and allow for the introduction of a polar residue at C-132. This is supported by the observation of side reactions of the endogenous 'monomeric' bacteriochlorophylls within the reaction center pigments, e.g. epimerization and hydroxylation at C-132. © 1990.

引用

页码：385 / 388

页数：4

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