INHIBITION BY TAURINE OF THE PHOSPHORYLATION OF SPECIFIC SYNAPTOSOMAL PROTEINS IN THE RAT CORTEX - EFFECTS OF TAURINE ON THE STIMULATION OF CALCIUM-UPTAKE IN MITOCHONDRIA AND INHIBITION OF PHOSPHOINOSITIDE TURNOVER

It has been previously observed that taurine inhibits PKC-activated phosphorylation of specific proteins including a approximately 20k M(r) protein in rat cortical synaptosomes. In the present study, the mechanism of the above effects of taurine were investigated. In an intrasynaptosomal cytosol fraction obtained by subcellular fractionation, taurine did not have inhibitory effects on protein phosphorylation. However, taurine did inhibit the phosphorylation of the approximately 20k M(r) protein in a reconstituted preparation containing intrasynaptosomal cytosol and mitochondria. Experiments measuring calcium uptake demonstrated that taurine increased the accumulation of (Ca2+)-Ca-45 in the mitochondrial fraction in incubation systems both in the absence and presence of added ATP. In addition, taurine inhibited the accumulation of P-32-labeled phosphatidic acid in synaptosomes indicative of a reduction in the levels of diacylglycerol. These results suggest that taurine may inhibit specific protein phosphorylation both by reducing cytosolic calcium levels and by inhibiting the turnover of phosphoinositides. These effects of taurine on the signal transduction cascade involving PKC and phosphoinositide metabolism indicate a potential biological role for taurine in the nervous system.