ANALYSIS OF SIALIC ACID-CONTAINING MUCIN OLIGOSACCHARIDES FROM PORCINE SMALL-INTESTINE BY HIGH-TEMPERATURE GAS-CHROMATOGRAPHY MASS-SPECTROMETRY OF THEIR DIMETHYLAMIDES

被引：18

作者：

HANSSON, GC ^{[1
]}

BOUHOURS, JF ^{[1
]}

KARLSSON, H ^{[1
]}

CARLSTEDT, I ^{[1
]}

机构：

[1] UNIV LUND, DEPT PHYSIOL CHEM 2, S-22100 LUND, SWEDEN

来源：

CARBOHYDRATE RESEARCH | 1991年 / 221卷

关键词：

D O I：

10.1016/0008-6215(91)80055-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acetylation of sialic acid-containing oligosaccharides lactonises the sialic acid residue quantitatively for all oligosaccharides studied except for 6'-sialyl-lactose. The modified, unsulphated, sialylated and sulphated oligosaccharides can then be fractionated by anion-exchange chromatography. Ammonolysis of the lactones followed by methylation yielded the dimethylamides, which are amenable to g.l.c.-m.s. and give intense and informative mass spectra. This approach has been used to characterise the sialic acid-containing O-linked oligosaccharides obtained from the mucin glycopeptides of the small intestine of the pig. At least 28 structures were found, having NeuAc or NeuGc 6-linked to the HexNAc attached to the peptide core or to a Hex 3-linked to HexNAc. Four different disialylated oligosaccharides were found having NeuAc or NeuGc on the Hex residue 3-linked to HexNAc and 6-linked to HexNAc.

引用

页码：179 / 189

页数：11

共 22 条

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