DNA CONFORMATIONAL CHANGE PRODUCED BY THE SITE-SPECIFIC INTERSTRAND CROSS-LINK OF TRANS-DIAMMINEDICHLOROPLATINUM(II)

被引：115

作者：

BRABEC, V ^{[1
]}

SIP, M ^{[1
]}

LENG, M ^{[1
]}

机构：

[1] CNRS, CTR BIOPHYS MOLEC, F-45071 ORLEANS 02, FRANCE

来源：

BIOCHEMISTRY | 1993年 / 32卷 / 43期

关键词：

D O I：

10.1021/bi00094a025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The DNA distortion produced by the interstrand cross-link of trans-diamminedichloroplatinum-(II) has been described by means of gel electrophoresis, chemical probes, and molecular mechanics modeling. Synthetic double-stranded oligodeoxyribonucleotides of varying lengths (19-22 base pairs) were synthesized that contained a unique site-specific interstrand cross-link within their central sequence d(TGCT)/d(AGCT) between complementary guanine and cytosine residues. We find that the platinated deoxyriboguanosine residue adopts syn conformation. The duplex is distorted on both sides of the cross-link, but the bases are still paired. The distortion introduces some flexibility into the helix. In addition, the double helix is unwound and bent toward the major groove.

引用

页码：11676 / 11681

页数：6

共 34 条

[31] THE MAJOR ADDUCT OF THE ANTITUMOR DRUG CIS-DIAMMINEDICHLOROPLATINUM(II) WITH DNA BENDS THE DUPLEX BY ALMOST-EQUAL-TO-40-DEGREES TOWARD THE MAJOR GROOVE [J].