ALKALOID METABOLISM IN TABERNAEMONTANA-DIVARICATA CELL-SUSPENSION CULTURES

被引：12

作者：

DAGNINO, D

SCHRIPSEMA, J

VERPOORTE, R

机构：

[1] Biotechnology Delft-Leiden, Project Group Plant Cell Biotechnology, Division of Pharmacognosy, Center for Bio-Pharmaceutical Sciences, Leiden University, Gorlaeus Laboratories, P.O. Box 9502

来源：

PHYTOCHEMISTRY | 1993年 / 32卷 / 02期

关键词：

TABERNAEMONTANA-DIVARICATA; APOCYNACEAE; INDOLE ALKALOID; O-ACETYLVALLESAMINE; PLANT CELL CULTURE; BIOSYNTHESIS; TURNOVER; CATABOLISM; N-15; LABELING;

D O I：

10.1016/S0031-9422(00)94989-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

O-Acetylvallesamine is the main alkaloid found in both medium and cells of the Tabernaemontana divaricata cell suspension culture investigated. [N-15]O-Acetylvallesamine was added to the culture medium. Rapid exchange between biomass and culture medium was shown by the rapid distribution of labelled compound over the two compartments. The occurrence of biosynthesis throughout most of the investigated period was confirmed by the observed dilution of the labelled compound. Transformation, calculated from the decrease in the total amount of [N-15]O-acetylvallesamine, was also observed and occurred mainly during the stationary phase of the batch cell culture growth cycle.

引用

页码：325 / 329

页数：5

共 10 条

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