FREQUENCY OF METASTASIS IN SYRIAN-HAMSTER TUMOR-CELLS SELECTED FOR LOW-LEVELS OF TYPICAL MULTIDRUG-RESISTANCE

被引：13

作者：

SHTIL, A ^{[1
]}

SHUSHANOV, A ^{[1
]}

MOYNOVA, E ^{[1
]}

STAVROVSKAYA, A ^{[1
]}

机构：

[1] CANC RES CTR,INST CARCINOGENESIS,REGULAT VIRAL & CELLULAR ONCOGENES LAB,MOSCOW,RUSSIA

来源：

EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY | 1994年 / 46卷 / 03期

关键词：

METASTASIS; RESISTANCE; MULTIDRUG; MULTIDRUG RESISTANCE;

D O I：

10.1016/S0940-2993(11)80096-5

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

The metastatic capability of cells at the initial stages of selection for ''typical'' (P-glycoprotein-mediated) multidrug resistance (MDR) was studied. Two independent sublines, 2SC/4-1 and 2SC/4-2 (11-12.4-fold resistant), and their 23- to 23.7-fold resistant 2SC/20-1 and 2SC/20-2 variants were isolated from highly tumorigenic (TrD(50) = 10 cells) and highly metastatic Rous sarcoma, virus-transformed Syrian hamster fibroblast HET-SR-2SC-LNM line for resistance to colchicine. 2SC/4 cells were less tumorigenic (TrD(50) = 70 cells) but as highly metastatic as parental counterparts. In contrast, both 2SC/20 variants showed a decrease in tumorigenicity (TrD(50) = 320 cells) and in the capability to produce spontaneous distant metastases. 2SC/20 cells almost lost the ability to colonize lungs in ex perimental metastasis assay. The autophosphorylation of pp60(src) tyrosine kinase in 2SC/20 cells was unaltered. The results suggest that i) selection of tumor cells for low levels of ''typical'' MDR leads to a decrease in the frequency of spontaneous and experimental metastases, and ii) alterations of malignancy in these cells are not caused by an impairment of function of a transforming oncogene.

引用

页码：257 / 262

页数：6

共 25 条

[11]

GANAPATHI R, 1987, CANCER RES, V47, P3554

[12]

GIAVAZZI R, 1983, CANCER RES, V43, P5081

[13]

HANANIA N, 1991, ANTICANCER RES, V11, P473

[14] MOLECULAR ASPECTS OF THE METASTATIC CASCADE [J].

HART, IR ;

GOODE, NT ;

WILSON, RE .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 989 (01) :65-84

[15]

KINSELLA AR, 1991, CANCER RES, V51, P1855

[16] THE APPROACHING ERA OF THE TUMOR SUPPRESSOR GENES [J].

KLEIN, G .

SCIENCE, 1987, 238 (4833) :1539-1545

[17] MULTIPLE SH2-MEDIATED INTERACTIONS IN V-SRC-TRANSFORMED CELLS [J].

KOCH, CA ;

MORAN, MF ;

ANDERSON, D ;

LIU, X ;

MBAMALU, G ;

PAWSON, T .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (03) :1366-1374

[18] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[19]

MOSSMANN T, 1983, J IMMUNOL METHODS, V65, P55

[20] ORGAN SPECIFICITY OF TUMOR-METASTASIS - ROLE OF PREFERENTIAL ADHESION, INVASION AND GROWTH OF MALIGNANT-CELLS AT SPECIFIC SECONDARY SITES [J].

NICOLSON, GL .

CANCER AND METASTASIS REVIEWS, 1988, 7 (02) :143-188

← 1 2 3 →