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DELTA-PH-DEPENDENT ACCUMULATION OF TETRACYCLINE IN ESCHERICHIA-COLI

被引：81
作者：
YAMAGUCHI, A
OHMORI, H
KANEKOOHDERA, M
NOMURA, T
SAWAI, T
机构：
[1] Div. of Microbial Chemistry, Fac. of Pharmaceut. Sciences, Chiba University
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1991年 / 35卷 / 01期
关键词：
D O I：
10.1128/AAC.35.1.53
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The effects of ionophores on tetracycline accumulation in Escherichia coli cells were investigated in the presence of polymyxin B nonapeptide. Accumulation was inhibited by nigericin but not by valinomycin. Tetracycline accumulation was stimulated by decreasing the pH of the medium and inhibited by the addition of magnesium ions. These results indicated that tetracycline enters cells through diffusion as a protonated form (TH2) and is accumulated as a membrane-impermeable magnesium-tetracycline chelate complex (THMg+). This noncarrier diffusion hypothesis was confirmed by the fact that tetracycline accumulated in protein-free liposomes through an artificially imposed pH difference.
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收藏
页码：53 / 56
页数：4
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