COMPLEMENT-DEPENDENT LYSIS OF CULTURED RAT MYOTUBES BY MYASTHENIC IMMUNOGLOBULINS

被引：16

作者：

ASHIZAWA, T ^{[1
]}

APPEL, SH ^{[1
]}

机构：

[1] VET ADM MED CTR, HOUSTON, TX 77211 USA

来源：

NEUROLOGY | 1985年 / 35卷 / 12期

关键词：

D O I：

10.1212/WNL.35.12.1748

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

We used cultured myotubes to demonstrate complement-dependent lysis of muscle membranes by serum from patients with myasthenia gravis. Lysis was monitored by light microscopy and release of incorporated [86Rb]. In the presence of guinea pig complement (GPC), 18 of 37 heat-inactivated myasthenic sera (49%), but none of 16 controls, caused morphologically detectable myotube lysis. Ten of 19 myasthenic sera (53%) increase [86Rb]-release compared with 10 controls. Immunoglobulin fractions retained the complement-dependent lytic activity. Inactivation of GPC prevented the lysis. [86Rb]-release appeared to correlate with clinical severity. The complement-dependent lysis resulted in a decrease in the number of acetylcholine receptors (AChRs) in myotubes, and AChR-immunoglobulin complexes were found in the medium of lysed cultures. The data suggest that cultured myotubes can be used to document complement-dependent antibody reactions in the pathogenesis of myasthenia.

引用

页码：1748 / 1753

页数：6