SHORT-CHAIN FLAVOR ESTER SYNTHESIS BY IMMOBILIZED LIPASE IN ORGANIC MEDIA

被引：111

作者：

MANJON, A ^{[1
]}

IBORRA, JL ^{[1
]}

AROCAS, A ^{[1
]}

机构：

[1] UNIV MURCIA,FAC CHEM,DEPT BIOCHEM & MOLEC BIOL,E-30001 MURCIA,SPAIN

来源：

BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 05期

关键词：

D O I：

10.1007/BF01027679

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Mucor miehei lipase has been adsorbed on Celite and covalently bound to nylon. The obtained derivatives have been studied regarding their ability for synthetize several flavouring esters in biphasic aqueous/organic media. The influence of the immobilization procedure on the synthetic activity of the derivatives was considered. Solvent hydrophobicity and water content in the biphasic system influenced both enzyme stability and equilibrium displacements. In this way, solvents with log P > 3.5 and less than 1% water were optimal. It was important to consider pH effects on enzyme microenvironment when using acidic substrates. Optimum temperature and reuse of the catalyst were also checked.

引用

页码：339 / 344

页数：6

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