STRUCTURAL CHARACTERIZATION OF BACITRACIN COMPONENTS BY FRIT-FAST ATOM BOMBARDMENT (FAB) LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY (LC/MS)

被引：22

作者：

IKAI, Y ^{[1
]}

OKA, H ^{[1
]}

HAYAKAWA, J ^{[1
]}

HARADA, K ^{[1
]}

SUZUKI, M ^{[1
]}

机构：

[1] MEIJO UNIV,FAC PHARM,TEMPAKU KU,NAGOYA,AICHI 468,JAPAN

来源：

JOURNAL OF ANTIBIOTICS | 1992年 / 45卷 / 08期

关键词：

D O I：

10.7164/antibiotics.45.1325

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The structural characterization of minor components of bacitracin (BC) complex was carried out using a technique of liquid chromatography/mass spectrometry (LC/MS). Satisfactory total ion current chromatogram of BC complex and excellent mass spectra of many components were given by Frit-fast atom bombardment (FAB) LC/MS analytical system, and the structures of 13 minor components could be proposed. The 13 minor components were classified into two groups, bacitracin A (BC-A) related components and bacitracin F (BC-F) related components depending on their common N-terminal moieties. The structures of BC-A related components and BC-F related components were the same as those of BC-A and BC-F, respectively, except that one to three of isoleucine and leucine residues are replaced by valines. The BC-F related components were degradation products of BC-A related components through the same degradation process as that of BC-A.

引用

页码：1325 / 1334

页数：10

共 26 条

[21] SOME PROPERTIES OF THE BACITRACIN POLYPEPTIDES
NEWTON, GGF
ABRAHAM, EP
[J]. BIOCHEMICAL JOURNAL, 1953, 53 (04) : 597 - 604
[22] IMPROVEMENT OF CHEMICAL-ANALYSIS OF ANTIBIOTICS .14. IDENTIFICATION OF THE COMPONENTS OF BACITRACIN USING NORMAL-PHASE AND REVERSED-PHASE THIN-LAYER CHROMATOGRAPHY
OKA, H
IKAI, Y
KAWAMURA, N
YAMADA, M
HARADA, KI
YAMAZAKI, Y
SUZUKI, M
[J]. JOURNAL OF CHROMATOGRAPHY, 1988, 449 (02): : 448 - 454
[23] IMPROVEMENT OF CHEMICAL-ANALYSIS OF ANTIBIOTICS .15. ISOCRATIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHODS FOR THE ANALYSIS AND PREPARATIVE SEPARATION OF THE COMPONENTS OF BACITRACIN
OKA, H
IKAI, Y
KAWAMURA, N
YAMADA, M
HARADA, K
YAMAZAKI, Y
SUZUKI, M
[J]. JOURNAL OF CHROMATOGRAPHY, 1989, 462 : 315 - 322
[24] RATTI E, 1967, J ORG CHEM, V33, P1309
[25] IDENTIFICATION OF ASPARAGINYL AND GLUTAMINYL RESIDUES IN ENDO POSITION IN PEPTIDES BY DEHYDRATION-REDUCTION
RESSLER, C
KASHELIK.DV
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1966, 88 (09) : 2025 - &
[26] IMPROVED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR POLYPEPTIDE ANTIBIOTICS AND ITS APPLICATION TO STUDY EFFECTS OF TREATMENTS TO REDUCE MICROBIAL LEVELS IN BACITRACIN POWDER
TSUJI, K
ROBERTSON, JH
[J]. JOURNAL OF CHROMATOGRAPHY, 1975, 112 (OCT29): : 663 - 672

← 1 2 3 →