CYTOKINE REGULATION OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR AND MACROPHAGE-COLONY-STIMULATING FACTOR PRODUCTION IN HUMAN ARTERIAL SMOOTH-MUSCLE CELLS

被引:71
作者
FILONZI, EL [1 ]
ZOELLNER, H [1 ]
STANTON, H [1 ]
HAMILTON, JA [1 ]
机构
[1] UNIV MELBOURNE,ROYAL MELBOURNE HOSP,DEPT MED,MELBOURNE,VIC 3050,AUSTRALIA
基金
英国医学研究理事会;
关键词
SMOOTH MUSCLE CELLS; COLONY STIMULATING FACTORS; CYTOKINES;
D O I
10.1016/0021-9150(93)90026-Q
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Smooth muscle cells (SMC) are the major cell type found in the walls of large blood vessels and appear to participate in local immune and inflammatory reactions, as well as in certain vascular diseases. We tested whether human arterial SMC can produce in vitro the colony stimulating factors (CSFs), granulocyte macrophage-CSF (GM-CSF) and macrophage CSF (M-CSF). Untreated internal mammary artery and aortic SMC produced no detectable GM-CSF but constitutively made M-CSF, measured by ELISA and radioimmunoassay, respectively. Interleukin-1 (IL-1) and, to a lesser extent, tumor necrosis factor alpha (TNFalpha) stimulated GM-CSF formation within 3 h; mRNA levels also increased particularly in the presence of the protein synthesis inhibitor, cycloheximide. IL-1, TNFalpha and, in addition, interferon-gamma (IFN-gamma) raised the M-CSF levels within 6 h; cycloheximide potentiated the effects of IL-1 and TNFalpha on mRNA levels. These results suggest that cytokine-stimulated human arterial SMC may be a source of the M-CSF found in atherosclerotic lesions. Since monocytes/macrophages can be activated by GM-CSF and M-CSF, while GM-CSF can also affect granulocyte function, SMC may participate in inflammatory reactions and vascular diseases by releasing these cytokines.
引用
收藏
页码:241 / 252
页数:12
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