AN INDUCIBLE EXPRESSION SYSTEM FOR THE PRODUCTION OF HUMAN LACTOFERRIN IN ASPERGILLUS-NIDULANS

被引：42

作者：

WARD, PP

MAY, GS

HEADON, DR

CONNEELY, OM

机构：

[1] BAYLOR COLL MED,DEPT CELL BIOL,1 BAYLOR PLAZA,HOUSTON,TX 77030

[2] NATL UNIV IRELAND UNIV COLL GALWAY,DEPT BIOCHEM,CELL & MOLEC BIOL GRP,GALWAY,IRELAND

来源：

GENE | 1992年 / 122卷 / 01期

关键词：

RECOMBINANT DNA; IRON-BINDING GLYCOPROTEIN; FILAMENTOUS FUNGI; ALCA PROMOTER; SECRETION; FUNCTIONAL ASSAY;

D O I：

10.1016/0378-1119(92)90054-S

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The production and secretion of human lactoferrin (hLF) in Aspergillus nidulans is described. The hLF cDNA was expressed under the control of the strong ethanol-inducible alcohol dehydrogenase (alcA) promoter. Recombinant hLF (re-hLF) is produced at levels up to 5 mug/ml. Approximately 30% of the re-hLF produced in this system is secreted into the growth medium. The re-hLF is indistinguishable from native hLF with respect to size and immunoreactivity. Furthermore, re-hLF is functional by the criterion of iron-binding capacity. The A. nidulans expression system offers an inexpensive, convenient method for the controlled production of mg amounts of biologically active mammalian glycoproteins.

引用

页码：219 / 223

页数：5

共 27 条

[11] AN IRON-BINDING PROTEIN COMMON TO MANY EXTERNAL SECRETIONS [J].