DETECTION OF TICK-BORNE ENCEPHALITIS-VIRUS RNA IN TICKS (IXODES-RICINUS) BY THE POLYMERASE CHAIN-REACTION

被引：34

作者：

RAMELOW, C

SUSS, J

BERNDT, D

ROGGENDORF, M

SCHREIER, E

机构：

[1] FED HLTH OFF,INST VET MED,DIEDERSDORFER WEG 1,D-12277 BERLIN 48,GERMANY

[2] FED HLTH OFF,ROBERT KOCH INST,BERLIN,GERMANY

[3] UNIV ESSEN GESAMTHSCH,INST VIROL,W-4300 ESSEN 1,GERMANY

来源：

JOURNAL OF VIROLOGICAL METHODS | 1993年 / 45卷 / 01期

关键词：

PCR; SOUTHERN BLOTTING; FLAVIVIRIDAE; TICK-BORNE ENCEPHALITIS VIRUS;

D O I：

10.1016/0166-0934(93)90145-H

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A polymerase chain reaction for the detection of tick-borne encephalitis virus (TBEV) RNA in ticks was developed. Two pairs of primers for nested PCR were selected from the 5'-NCR and the 5'-terminus of the C protein coding region, which are highly conserved among the TBEV isolates sequenced so far. The sensitivity of the nested PCR was tested by dilution experiments of a TBEV positive brain suspension. The specificity of the PCR products was confirmed by Southern blotting. In a pilot study, 60 homogenates of 7200 ticks (I. ricinus) were examined by PCR. Two homogenates were found positive. The PCR for TBEV RNA appears to be a valuable method to define endemic areas of TBE.

引用

页码：115 / 119

页数：5