FLUORESCENCE PARTICLE COUNTER FOR DETECTING AIRBORNE BACTERIA AND OTHER BIOLOGICAL PARTICLES

被引:106
作者
PINNICK, RG
HILL, SC
NACHMAN, P
PENDLETON, JD
FERNANDEZ, GL
MAYO, MW
BRUNO, JG
机构
[1] NEW MEXICO STATE UNIV, DEPT PHYS, LAS CRUCES, NM 88003 USA
[2] USAF, ARMSTRONG LAB,EDGEWOOD RES DEV & ENGN CTR, ABERDEEN PROVING GROUND, MD 21010 USA
关键词
D O I
10.1080/02786829508965345
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
We have constructed a laser-based particle counter that detects the fluorescence, as well as the elastic scattering, from individual airborne particles as they traverse a laser beam. This fluorescence particle counter (FPC) can detect fluorescence from mu m-sized Bacillus subtilis spore agglomerates when illuminated with intense light at 488 nm from an argon ion laser, either similar to 0.7 kW cm(-2) extracavity or similar to 50 kW cm(-2) intracavity. We suspect that flavins in the spores are the molecules primarily responsible for the fluorescence, because the peak fluorescence emission of the biological materials at this excitation wavelength is in the range 530-550 nm, which is characteristic of flavins. Fluorescence form kaolin, hematite, and polystyrene particles was not detectable, the lack of fluorescence indicates that the FPC may be able to differentiate between biological and nonbiological aerosols. The FPC samples aerosol-laden air at a rate of similar to 1 mL s(-1), and is capable of measuring aerosol concentrations up to several thousand per milliliter. The FPC may be helpful in detecting and characterizing airborne bacteria and other airborne particles of biological origin.
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收藏
页码:653 / 664
页数:12
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