DOPACHROME CONVERSION ACTIVITY IN AEDES-AEGYPTI - SIGNIFICANCE DURING MELANOTIC ENCAPSULATION OF PARASITES AND CUTICULAR TANNING

被引：31

作者：

LI, JY

ZHAO, XL

CHRISTENSEN, BM

机构：

[1] Department of Animal Health and Biomedical Sciences, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, WI 53706

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1994年 / 24卷 / 10期

关键词：

AEDES AEGYPTI; L-TYROSINE; L-DOPA; L-DOPACHROME; PHENOL OXIDASE; DOPACHROME CONVERSION ENZYME; MELANIZATION;

D O I：

10.1016/0965-1748(94)90142-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phenol oxidase (PO) and dopachrome conversion enzyme (DCE) were partially purified from Aedes aegypti larvae by ammonium sulfate fractionation. PO from A. aegypti functions in the hydroxylation of monophenols (e.g., tyrosine and tyramine) to their related o-diphenols, and the oxidation of o-diphenols (e.g., L-dopa, dopamine, N-acetyldopamine) to their respective o-quinones. Partially purified DCE showed high specificity toward dopachrome generated from dopa with the L-configuration. The combined effects of PO and DCE significantly accelerated melanization pathways when L-dopa was used as substrate. Significant DCE activity also was detected in hemolymph samples from adult, female A. aegypti, and undoubtedly plays a role in melanotic encapsulation reactions.

引用

页码：1043 / 1049

页数：7

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