CONSTRUCTION OF HIS(6)-TAGGING VECTORS ALLOWING SINGLE-STEP PURIFICATION OF GROES AND OTHER POLYPEPTIDES PRODUCED IN BACILLUS-SUBTILIS

被引：29

作者：

SCHON, U ^{[1
]}

SCHUMANN, W ^{[1
]}

机构：

[1] UNIV BAYREUTH,LEHRSTUHL GENET,D-95440 BAYREUTH,GERMANY

来源：

GENE | 1994年 / 147卷 / 01期

关键词：

RECOMBINANT DNA; EXPRESSION SHUTTLE VECTOR; IMINODIACETIC ACID-AGAROSE; NI-NITRILOTRIACETIC ACID AFFINITY CHROMATOGRAPHY; PCR; GENE GROES;

D O I：

10.1016/0378-1119(94)90044-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two plasmid expression vectors for Bacillus subtilis have been constructed that direct the synthesis of fusion proteins containing a stretch of six histidine residues (His(6)) at either the N or C terminus. The His(6) tag allows the rapid enrichment of proteins by metal chelate affinity chromatography in a denatured or native state. As an example of the general utility of one of these expression vectors, we produced the GroES protein encoded by the groESL operon of B. stearothermophilus.

引用

页码：91 / 94

页数：4

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