TANDEMLY REPEATED TRANSGENES OF THE HUMAN MINISATELLITE-MS32-(D1S8), WITH NOVEL MOUSE-GAMMA SATELLITE

被引:22
作者
ALLEN, MJ
JEFFREYS, AJ
SURANI, MA
BARTON, S
NORRIS, ML
COLLICK, A
机构
[1] WELLCOME CRC INST DEV BIOL,CAMBRIDGE CB2 1QR,CAMBS,ENGLAND
[2] AFRC,INST ANIM PHYSIOL & GENET,CAMBRIDGE CB2 4AT,CAMBS,ENGLAND
基金
英国惠康基金;
关键词
D O I
10.1093/nar/22.15.2976
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The human hypervariable minisatellite MS32 has a well characterised internal repeat unit array and high mutation rates have been observed at this locus. Analysis of MS32 mutants has shown that male germline mutations are polarised to one end of the array and frequently involve complex gene conversion-like events, suggesting that tandem repeat instability may be modulated by cis-acting sequences flanking the locus. In order to investigate the processes affecting MS32 mutation rate and mechanism, we have created transgenic mice harbouring an MS32 allele. Here we describe the organisation of eight transgenic insertions. Analysis of these transgenic loci by MVR-PCR and structural analysis of the junctions between mouse flanking DNA and the transgenic loci has shed light on mechanisms of integration and rearrangement of the tandem repeated transgenes. Sequence analysis of the moose DNA flanking these transgenes has shown that 5 of the 8 insertions have integrated into mouse gamma satellite repeated sequence. This suggets a non-random integration of the MS32 transgene construct into the mouse genome.
引用
收藏
页码:2976 / 2981
页数:6
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