Genetic probes of ribosomal RNA function

被引：52

作者：

OConnor, M

Brunelli, CA

Firpo, MA

Gregory, ST

Lieberman, KR

Lodmell, JS

Moine, H

VanRyk, DI

Dahlberg, AE

机构：

[1] Department of Molecular and Cellular Biology and Biochemistry, J. Wilson Laboratory, Brown University, Providence, 02912, RI

来源：

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1995年 / 73卷 / 11-12期

关键词：

decoding; peptidyltransferase; streptomycin; paromomycin; suppression; 45S RNA;

D O I：

10.1139/o95-093

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used a genetic approach to uncover the functional roles of rRNA in protein synthesis. Mutations were constructed in a cloned rrn operon by site-directed mutagenesis or isolated by genetic selections following random mutagenesis. We have identified mutations that affect each step in the process of translation. The data are consistent with the results of biochemical and phylogenetic analyses but, in addition, have provided novel information on regions of rRNA not previously investigated.

引用

页码：859 / 868

页数：10

共 55 条

[1] RIBOSOMAL COMPONENTS NEIGHBORING THE CONSERVED 518-533-LOOP OF 16S RIBOSOMAL-RNA IN 30S SUBUNITS [J].

ALEXANDER, RW ;

MURALIKRISHNA, P ;

COOPERMAN, BS .

BIOCHEMISTRY, 1994, 33 (40) :12109-12118

[2]

ATKINS JF, 1991, ANNU REV GENET, V25, P201

[3] MUTATIONS IN 23-S RIBOSOMAL-RNA PERTURB TRANSFER-RNA SELECTION AND CAN LEAD TO STREPTOMYCIN DEPENDENCE [J].

BILGIN, N ;

EHRENBERG, M .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 235 (03) :813-824

[4] INITIATION OF TRANSLATION IS IMPAIRED IN ESCHERICHIA-COLI-CELLS DEFICIENT IN 4.5S RNA [J].

BOURGAIZE, DB ;

FOURNIER, MJ .

NATURE, 1987, 325 (6101) :281-284

[5]

BRIMACOMBE R, 1995, RIBOSOMAL RNA STRUCT

[6] TIME OF ACTION OF 4-5-S RNA IN ESCHERICHIA-COLI TRANSLATION [J].

BROWN, S .

JOURNAL OF MOLECULAR BIOLOGY, 1989, 209 (01) :79-90

[7]

CUNDLIFFE E, 1990, RIBOSOME, P479

[8] RATES OF AMINOACYL-TRANS-RNA SELECTION AT 29 SENSE CODONS INVIVO [J].

CURRAN, JF ;

YARUS, M .

JOURNAL OF MOLECULAR BIOLOGY, 1989, 209 (01) :65-77

[9] MUTATIONS IN 16S RIBOSOMAL-RNA DISRUPT ANTIBIOTIC - RNA INTERACTIONS [J].

DESTASIO, EA ;

MOAZED, D ;

NOLLER, HF ;

DAHLBERG, AE .

EMBO JOURNAL, 1989, 8 (04) :1213-1216

[10] EFFECTS OF MUTAGENESIS OF A CONSERVED BASE-PAIRED SITE NEAR THE DECODING REGION OF ESCHERICHIA-COLI 16S RIBOSOMAL-RNA [J].

DESTASIO, EA ;

DAHLBERG, AE .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 212 (01) :127-133

← 1 2 3 4 5 6 →