Genetic probes of ribosomal RNA function

被引：52

作者：

OConnor, M

Brunelli, CA

Firpo, MA

Gregory, ST

Lieberman, KR

Lodmell, JS

Moine, H

VanRyk, DI

Dahlberg, AE

机构：

[1] Department of Molecular and Cellular Biology and Biochemistry, J. Wilson Laboratory, Brown University, Providence, 02912, RI

来源：

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1995年 / 73卷 / 11-12期

关键词：

decoding; peptidyltransferase; streptomycin; paromomycin; suppression; 45S RNA;

D O I：

10.1139/o95-093

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used a genetic approach to uncover the functional roles of rRNA in protein synthesis. Mutations were constructed in a cloned rrn operon by site-directed mutagenesis or isolated by genetic selections following random mutagenesis. We have identified mutations that affect each step in the process of translation. The data are consistent with the results of biochemical and phylogenetic analyses but, in addition, have provided novel information on regions of rRNA not previously investigated.

引用

页码：859 / 868

页数：10

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