NEUROTROPHIN-3 RESCUES NEURONAL PRECURSORS FROM APOPTOSIS AND PROMOTES NEURONAL DIFFERENTIATION IN THE EMBRYONIC METANEPHRIC KIDNEY

被引：36

作者：

KARAVANOV, A

SAINIO, K

PALGI, J

SAARMA, M

SAXEN, L

SARIOLA, H

机构：

[1] UNIV HELSINKI, DEPT PATHOL, SF-00014 HELSINKI, FINLAND

[2] UNIV HELSINKI, INST BIOTECHNOL, SF-00014 HELSINKI, FINLAND

[3] UNIV HELSINKI, CHILDRENS HOSP, SF-00290 HELSINKI, FINLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 24期

关键词：

METANEPHROS; KIDNEY DIFFERENTIATION; NEUROTROPHIN RECEPTOR;

D O I：

10.1073/pnas.92.24.11279

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We analyzed the developmental regulation and role of the neurotrophins during metanephric kidney morphogenesis. RNase protection assay revealed the presence of nerve growth factor, neurotrophin 3 (NT-3), and brain-derived neurotrophic factor mRNAs and the regulation of their expression during embryonic development of rat metanephros. NT-3 induced differentiation (neurite outgrowth) and survival (inhibition of apoptosis) of the neuronal precursors in cultured nephrogenic mesenchymes and neuronal differentiation in cultured whole kidneys, whereas NT-4/5, brain-derived neurotrophic factor, and nerve growth factor were without effect. The neurotrophins did not trigger tubular differentiation of isolated nephrogenic cells, which underwent apoptosis when cultured with or without the neurotrophins. NT-3 is thus an inducer of differentiation and a survival factor for renal neuronal cells, but none of the neurotrophins is a morphogen in kidney tubule induction.

引用

页码：11279 / 11283

页数：5

共 44 条

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