CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF DIALKYLGLYCINE DECARBOXYLASE, A DECARBOXYLATING TRANSAMINASE

被引：4

作者：

TONEY, MD ^{[1
]}

KELLER, JW ^{[1
]}

PAUPTIT, RA ^{[1
]}

JAEGER, J ^{[1
]}

WISE, MK ^{[1
]}

SAUDER, U ^{[1
]}

JANSONIUS, JN ^{[1
]}

机构：

[1] UNIV ALASKA FAIRBANKS,DEPT CHEM,FAIRBANKS,AK 99775

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 222卷 / 04期

关键词：

TRANSAMINATION; X-RAY CRYSTALLOGRAPHY; DECARBOXYLATION; CRYSTALLIZATION; VITAMIN-B6;

D O I：

10.1016/0022-2836(91)90580-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pyridoxal phosphate-dependent enzyme dialkylglycine decarboxylase (E.C. 4.1.1.64) has been crystallized by vapor diffusion from a 15% polyethyleneglycol solution with sodium pyruvate as coprecipitant. The space group of the crystals is either P6222 or the enantiomorph, P6422, with one subunit of 46,500 Da per asymmetric unit. The unit cell has dimensions a = b = 152·7 A ̊, c = 86·6 A ̊, α = β = 90 °, γ = 120 °, and a solvent content of approximately 61%. Diffraction extends to 2·3 Å resolution. © 1991.

引用

页码：873 / 875

页数：3

共 15 条

[11] CRYSTAL ORIENTATION AND X-RAY PATTERN PREDICTION ROUTINES FOR AREA-DETECTOR DIFFRACTOMETER SYSTEMS IN MACROMOLECULAR CRYSTALLOGRAPHY [J].