NO ALLELIC LOSS AT THE P53 LOCUS IN 1,2-DIMETHYLHYDRAZINE-INDUCED MOUSE COLON TUMORS - PCR SSCP ANALYSIS WITH SEQUENCE-TAGGED MICROSATELLITE SITE PRIMERS

被引：30

作者：

OKAMOTO, M

OHTSU, H

MIYAKI, M

YONEKAWA, H

机构：

[1] TOKYO METROPOLITAN INST MED SCI,DEPT BIOCHEM,TOKYO 113,JAPAN

[2] NATL INST RADIOL SCI,DEPT PHYSIOL & PATHOL,INAGE KU,CHIBA 260,JAPAN

来源：

CARCINOGENESIS | 1993年 / 14卷 / 07期

关键词：

D O I：

10.1093/carcin/14.7.1483

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We examined allelic loss in colon tumors induced by 1,2-dimethylhydrazine (DMH) in F1 hybrid mice, using sequence-tagged microsatellite site (STMS) primers derived from the chromosomal region closely linked to the p53 locus. Polymerase chain reaction-single-strand conformation polymorphism (PCR - SSCP) analysis of 155 colonic tumors with two STMS markers revealed that no genetic alterations had occurred in these tumors, except for one case where one of the markers detected an increase of one CA repeat unit in one allele. No allelic loss at the loci closely linked to the p53 locus strongly suggests that allelic loss at the p53 locus is not involved in DMH-induced colon carcinogenesis in mice.

引用

页码：1483 / 1486

页数：4

共 35 条

[1] MONONUCLEOTIDE REPEATS ARE AN ABUNDANT SOURCE OF LENGTH VARIANTS IN MOUSE GENOMIC DNA [J].