PURIFICATION AND PARTIAL CHARACTERIZATION OF A LYSINE-SPECIFIC PROTEASE OF PORPHYROMONAS-GINGIVALIS

被引：18

作者：

FUJIMURA, S

SHIBATA, Y

NAKAMURA, T

机构：

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 113卷 / 02期

关键词：

PORPHYROMONAS-GINGIVALIS; PROTEASE;

D O I：

10.1111/j.1574-6968.1993.tb06503.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A lysine-specific protease hydrolysing peptide bonds at the carboxyl side of lysine residues in Porphyromonas gingivalis was purified from culture supernatant by a combination of ion-exchange chromatography, gel filtration, and affinity chromatography. The molecular mass was 48 kDa and the pI value was 7.3. The enzyme hydrolysed the peptide bonds at the carboxyl side of lysine residues in synthetic substrates and natural proteins.

引用

页码：133 / 138

页数：6

共 21 条

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