FUNCTIONAL IDENTIFICATION OF THE PROMOTER FOR THE GENE ENCODING THE ALPHA-SUBUNIT OF CALCIUM CALMODULIN-DEPENDENT PROTEIN-KINASE-II

被引:19
作者
OLSON, NJ
MASSE, T
SUZUKI, T
CHEN, J
ALAM, D
KELLY, PT
机构
[1] Dept. of Neurobiology and Anatomy, University of Texas, Houston
关键词
D O I
10.1073/pnas.92.5.1659
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
To examine the expression of the alpha subunit of calcium/calmodulin-dependent protein kinase II, various 5' flanking genomic sequences were inserted into a chloramphenicol acetyltransferase (CAT) reporter plasmid and CAT enzyme activities were analyzed in transfected NB2a neuroblastoma cells and mRNA transcription was analyzed by nuclease protection assays. A core promoter was identified which contained an essential TATA element located 162 nt 5' to the transcription start site. Sequences 3' to the transcription start site, as well as 5' to the TATA element, increased levels of CAT activity in transfected cells. The a-subunit gene promoter displayed higher CAT activities, relative to a simian virus 40 promoter, in transfected neuronal cell lines than in nonneuronal cell lines. Results also suggested that sequence surrounding the natural alpha-gene transcription initiation site may be important for targeting transcription initiation 162 nt downstream of its TATA element.
引用
收藏
页码:1659 / 1663
页数:5
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