DETECTION OF FOSFOMYCIN RESISTANCE BY THE POLYMERASE CHAIN-REACTION AND WESTERN BLOTTING

被引：8

作者：

GARCIA, P ^{[1
]}

ARCA, P ^{[1
]}

TOYOS, JR ^{[1
]}

SUAREZ, JE ^{[1
]}

机构：

[1] UNIV OVIEDO,FAC MED,AREA MICROBIOL,E-33006 OVIEDO,SPAIN

来源：

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 1994年 / 34卷 / 06期

关键词：

D O I：

10.1093/jac/34.6.955

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The polymerase chain reaction and Western blotting were assessed as means of detecting plasmid-encoded fosfomycin resistance in the hope that they might facilitate epidemiological studies. The results indicated good correlation between the two methods which are more rapid, less expensive and more sensitive than DNA-DNA hybridization. Both techniques could potentially be employed for the genotypic detection of resistance to any group of antibiotics.

引用

页码：955 / 963

页数：9

共 22 条

[1] DISPERSION OF A GENE THAT CODIFIES FOSFOMYCIN RESISTANCE AMONG PLASMIDS FROM ENTEROBACTERIACEAE ISOLATED FROM SEWAGE
ALVAREZ, AM
HARDISSON, C
MENDOZA, MC
[J]. FEMS MICROBIOLOGY LETTERS, 1987, 48 (03) : 351 - 356
[2] FORMATION OF AN ADDUCT BETWEEN FOSFOMYCIN AND GLUTATHIONE - A NEW MECHANISM OF ANTIBIOTIC-RESISTANCE IN BACTERIA
ARCA, P
RICO, M
BRANA, AF
VILLAR, CJ
HARDISSON, C
SUAREZ, JE
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1988, 32 (10) : 1552 - 1556
[3] PURIFICATION OF A GLUTATHIONE S-TRANSFERASE THAT MEDIATES FOSFOMYCIN RESISTANCE IN BACTERIA
ARCA, P
HARDISSON, C
SUAREZ, JE
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1990, 34 (05) : 844 - 848
[4] GENOTYPIC APPROACH TO THE STUDY OF BACTERIAL-RESISTANCE TO ANTIBIOTICS
COURVALIN, P
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1991, 35 (06) : 1019 - 1023
[5] SPECIES-SPECIFIC DETECTION OF LISTERIA-MONOCYTOGENES BY DNA AMPLIFICATION
DENEER, HG
BOYCHUK, I
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (02) : 606 - 609
[6] Erlich H. A., 1989, PCR TECHNOLOGY PRINC
[7] GAYLORD H, 1987, ANNU REV MICROBIOL, V41, P645
[8] ISOLATION AND CHARACTERIZATION OF MUTATIONS AFFECTING TRANSPORT OF HEXOSE PHOSPHATES IN ESCHERICHIA-COLI
KADNER, RJ
WINKLER, HH
[J]. JOURNAL OF BACTERIOLOGY, 1973, 113 (02) : 895 - 900
[9] MECHANISM OF ACTION OF FOSFOMYCIN (PHOSPHONOMYCIN)
KAHAN, FM
KAHAN, JS
CASSIDY, PJ
KROPP, H
[J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1974, 235 (MAY10) : 364 - 386
[10] Kestle D G, 1969, Antimicrob Agents Chemother (Bethesda), V9, P332

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