HORMONAL-CONTROL OF FEMALE SEX-PHEROMONE BIOSYNTHESIS IN THE REDBANDED LEAFROLLER MOTH, ARGYROTAENIA-VELUTINANA

被引:57
作者
JURENKA, RA [1 ]
FABRIAS, G [1 ]
ROELOFS, WL [1 ]
机构
[1] CORNELL UNIV,NEW YORK STATE AGR EXPT STN,DEPT ENTOMOL,GENEVA,NY 14456
来源
INSECT BIOCHEMISTRY | 1991年 / 21卷 / 01期
基金
美国国家科学基金会;
关键词
SYNTHETIC; PBAN; NEUROPEPTIDE; BRAIN; CORPUS BURSAE EXTRACTS; ABDOMEN CULTURE; RADIOLABELED ACETATE;
D O I
10.1016/0020-1790(91)90067-O
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pheromone biosynthesis in female redbanded leafroller moths (RBLR) is under control of a neuropeptide produced in the brain. A bioassay consisting of isolated abdomens was developed to test the mode of action of the pheromone biosynthesis activating neuropetide (PBAN). Pheromone titer and incorporation of radiolabeled acetate into pheromone could be monitored with this bioassay. Synthetic PBAN with sequences identical to PBAN isolated from Heliothis zea and Bombyx mori were active in inducing synthesis of pheromone in RBLR. Removal of the ventral nerve cord in isolated abdomens did not inhibit the action of PBAN. Small amounts of PBAN-like activity was found in hemolymph collected from normal females but not from decapitated females. Severing the VNC in vivo in normal females did not lower the pheromone titer. These data indicate that PBAN is released into the hemolymph and then travels to its site of action. A two-fold increase in both pheromone titer and radiolabeled acetate incorporation upon incubation with PBAN was shown with isolated pheromone glands. However, the differences between control and PBAN-induced values were smaller than those obtained with the isolated abdomen culture bioassay where a seven-fold increase was observed. A decrease in pheromone titer was seen upon the in vivo removal of the corpus bursae from normal females. Removal of the corpus bursae in the isolated abdomen cultures also abolished the activity of PBAN. However, cutting the cervix bursae and leaving the corpus bursae in the abdomen culture increased both titer and radiolabeled acetate incorporation into pheromone without the presence of PBAN. An aqueous extract made from the corpus bursae of 5-day-old females was also active by itself in inducing pheromone biosynthesis in the isolated abdomen cultures. Experiments performed using newly emerged females confirmed that the corpus bursae extracts will induce pheromone biosynthesis. These results indicate that both PBAN and the corpus bursae are involved in controlling pheromone biosynthesis in RBLR.
引用
收藏
页码:81 / 89
页数:9
相关论文
共 24 条
[21]   ENDOGENOUS SUPPRESSION OF PHEROMONE PRODUCTION IN VIRGIN FEMALE MOTHS [J].
TEAL, PEA ;
TUMLINSON, JH ;
OBERLANDER, H .
EXPERIENTIA, 1990, 46 (10) :1047-1050
[22]   NEURAL REGULATION OF SEX-PHEROMONE BIOSYNTHESIS IN HELIOTHIS MOTHS [J].
TEAL, PEA ;
TUMLINSON, JH ;
OBERLANDER, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (07) :2488-2492
[23]   THE EFFECTS OF MATING, EXOGENOUS JUVENILE-HORMONE AND A JUVENILE-HORMONE ANALOG ON PHEROMONE TITER, CALLING AND OVIPOSITION IN THE OMNIVOROUS LEAFROLLER MOTH (PLATYNOTA-STULTANA) [J].
WEBSTER, RP ;
CARDE, RT .
JOURNAL OF INSECT PHYSIOLOGY, 1984, 30 (02) :113-118
[24]  
YI Q, 1989, J XIAMEN U NAT SCI, V28, P88