THE PYRUVATE KINASE-COUPLED ASSAY FOR ATPASES - A CRITICAL ANALYSIS

被引：26

作者：

JENKINS, WT

机构：

[1] Department of Chemistry, Indiana University, Bloomington

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 194卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90160-U

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The pyruvate kinase assay for ATPases suffers from major systematic errors due to the variable amounts of the inhibitory product, ADP, present as the rates change. A simple method is presented to estimate the true uninhibited rates and the ADP inhibition constant for the ATPase. © 1991.

引用

页码：136 / 139

页数：4

共 15 条

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