ADJUSTMENT OF RESOLUTION AND ANALYSIS TIME IN CAPILLARY ZONE ELECTROPHORESIS BY VARYING THE PH OF THE BUFFER

An expression for resolution in capillary zone electrophoresis is derived which is a function of the pH of the buffer. It is based on the conventional definitions of resolution, but takes into account the dependence of the efficiency term on the effective mobilities, or the charge numbers, of the separands (monovalent ions). The different shapes of the R vs. pH curves, as derived from the resolution equation, are discussed and found to agree with the experimental results. An approach for the selection of that single pH where a given resolution can be achieved within the shortest time of analysis is introduced.