QUANTITATIVE-ANALYSIS OF PHOSPHOROTHIOATE OLIGONUCLEOTIDES IN BIOLOGICAL-FLUIDS USING DIRECT-INJECTION FAST ANION-EXCHANGE CHROMATOGRAPHY AND CAPILLARY GEL-ELECTROPHORESIS

被引：58

作者：

BOURQUE, AJ ^{[1
]}

COHEN, AS ^{[1
]}

机构：

[1] HYBRIDON,WORCESTER,MA 01605

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 662卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00207-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The analysis of antisense phosphorothioate DNA (SODN) in human plasma via direct injection using anion-exchange high-performance liquid chromatography (AE-HPLC) is presented. The method relies on the ability to selectively extract phosphorothioate DNA from undigested serum, plasma and urine on anion-exchange resins. The automated HPLC method can analyze a sample every 5 min with a limit of detection of 50 ng/ml (ppb). The DNA was collected, desalted and analyzed by capillary gel electrophoresis. Due to the high resolving power of this technique, a qualitative assessment of enzymatic degradation of the antisense oligonucleotide can be made.

引用

页码：343 / 349

页数：7

共 9 条

[1] PHARMACOKINETICS, BIODISTRIBUTION, AND STABILITY OF OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATES IN MICE
AGRAWAL, S
TEMSAMANI, J
TANG, JY
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (17) : 7595 - 7599
[2] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF PHOSPHOROTHIOATE ANALOGS OF OLIGODEOXYNUCLEOTIDES IN BIOLOGICAL-FLUIDS
BIGELOW, JC
CHRIN, LR
MATHEWS, LA
MCCORMACK, JJ
[J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 533 : 133 - 140
[3] QUANTITATIVE-ANALYSIS OF PHOSPHOROTHIOATE OLIGONUCLEOTIDES IN BIOLOGICAL-FLUIDS USING FAST ANION-EXCHANGE CHROMATOGRAPHY
BOURQUE, AJ
COHEN, AS
[J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1993, 617 (01): : 43 - 49
[4] RAPID SEPARATION AND PURIFICATION OF OLIGONUCLEOTIDES BY HIGH-PERFORMANCE CAPILLARY GEL-ELECTROPHORESIS
COHEN, AS
NAJARIAN, DR
PAULUS, A
GUTTMAN, A
SMITH, JA
KARGER, BL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (24) : 9660 - 9663
[5] COHEN AS, 1993, J CHROMATOGR, V638, P293
[6] MULTIPLE BINDING MODES OF THE SINGLE-STRANDED-DNA BINDING-PROTEIN FROM ESCHERICHIA-COLI AS DETECTED BY TRYPTOPHAN FLUORESCENCE AND SITE-DIRECTED MUTAGENESIS
CURTH, U
GREIPEL, J
URBANKE, C
MAASS, G
[J]. BIOCHEMISTRY, 1993, 32 (10) : 2585 - 2591
[7] ISOLATION OF HIGH-MOLECULAR-WEIGHT DNA FROM MAMMALIAN-CELLS
GROSS-BELLARD, MM
OUDET, P
CHAMBON, P
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1973, 36 (01): : 32 - 38
[8] SEPARATION OF DNA RESTRICTION FRAGMENTS BY HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS WITH LOW AND ZERO CROSS-LINKED POLYACRYLAMIDE USING CONTINUOUS AND PULSED ELECTRIC-FIELDS
HEIGER, DN
COHEN, AS
KARGER, BL
[J]. JOURNAL OF CHROMATOGRAPHY, 1990, 516 (01): : 33 - 48
[9] ONLINE INTERNAL SURFACE REVERSED-PHASE CLEANING - THE DIRECT HPLC ANALYSIS OF CRUDE BIOLOGICAL SAMPLES - APPLICATION TO THE KINETICS OF DEGRADATION OF OLIGONUCLEOTIDES IN CELL-CULTURE MEDIUM
POMPON, A
LEFEBVRE, I
IMBACH, JL
[J]. BIOCHEMICAL PHARMACOLOGY, 1992, 43 (08) : 1769 - 1775

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