QUANTITATIVE-ANALYSIS OF CYTOKININS BY ELECTROSPRAY TANDEM MASS-SPECTROMETRY

被引：72

作者：

PRINSEN, E ^{[1
]}

REDIG, P ^{[1
]}

VANDONGEN, W ^{[1
]}

ESMANS, EL ^{[1
]}

VANONCKELEN, HA ^{[1
]}

机构：

[1] UNIV ANTWERP,RUCA,DEPT CHEM,NUCLEOSIDE RES & MASS SPECTROMETRY UNIT,B-2020 ANTWERP,BELGIUM

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1995年 / 9卷 / 10期

关键词：

D O I：

10.1002/rcm.1290091016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A methodology was developed for the analysis of cytokinins from plant tissue using electrospray tandem mass spectrometry in combination with HPLC. A detection limit of 1 pmol zeatin-riboside injected on-column was achieved under multiple reaction monitoring conditions. Linearity was observed within a concentration range of 1-100 pmol injected on-column. In view of the analysis of a considerable amount of samples, the chromatographic conditions were selected which were a compromise between speed of analysis and resolution, This approach, in combination with multiple reaction monitoring, allowed the analysis of up to 150 samples a day, and quantitation of 16 different cytokinins per sample.

引用

页码：948 / 953

页数：6

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