HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF NATURALLY-OCCURRING FLAVONOIDS IN CITRUS WITH A PHOTODIODE-ARRAY DETECTOR

被引:83
作者
NOGATA, Y
OHTA, H
YOZA, KI
BERHOW, M
HASEGAWA, S
机构
[1] MINIST AGR FORESTRY & FISHERIES,CHUGOKU NATL AGR EXPT STN,FUKUYAMA,HIROSHIMA 721,JAPAN
[2] USDA ARS,FRUIT & VEGETABLE CHEM LAB,PASADENA,CA 91106
关键词
D O I
10.1016/0021-9673(94)89051-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
High-performance liquid chromatography (HPLC) coupled with ultraviolet-visible spectrophotometry using a photodiode-array detector was used as a routine method for the simultaneous separation and determination of 25 naturally occurring Citrus flavonoids. The separation system consisted of a C-18 reversed-phase column, a gradient system of 0.01 M phosphoric acid (A) and methanol (B), and a photodiode-array detector. Each of the 25 flavonoids was eluted from the column with a gradient system composed of three periods: (1) 0-55 min, 70-55% (v/v) A in B, (2) 55-95 min, 55-0% A in B, and (3) 95-100 min, isocratic, 100% B, and quantified by spectrophotometric detection at 285 nm. Identifications of specific flavonoids were made by comparing their retention times (t(R)) and UV spectra with those of standards. The relative standard deviations of t(R) values were 0.029-0.321%. The recoveries of pure eriocitrin, naringin, hesperidin and tangeretin added to tissues prepared from Unshiu (Citrus unshiu Marc.) and Hirado-buntan (Citrus grandis Osbeck f. Hirado) and subsequent extraction were 97.47-103.13% from the mesocarp and 96.87-104.93% from the juice with standard deviations of 2.32-5.72% and 2.18-5.96%, respectively.
引用
收藏
页码:59 / 66
页数:8
相关论文
共 30 条
[1]  
ANIS M, 1981, J PLANT BIOCH, V8, P56
[2]  
ARMAND J, 1988, PLANT FLAVONOIDS BIO, V2, P235
[3]   KINETICS OF THE INHIBITORY EFFECT OF FLAVONOIDS ON HISTAMINE-SECRETION FROM MAST-CELLS [J].
BRONNER, C ;
LANDRY, Y .
AGENTS AND ACTIONS, 1985, 16 (3-4) :147-151
[4]   FLAVONOID MODULATION OF HUMAN NEUTROPHIL FUNCTION [J].
BUSSE, WW ;
KOPP, DE ;
MIDDLETON, E .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1984, 73 (06) :801-809
[5]   HIGH-PERFORMANCE REVERSED-PHASE LIQUID-CHROMATOGRAPHY OF NATURALLY OCCURRING PHENOLIC COMPOUNDS [J].
COURT, WA .
JOURNAL OF CHROMATOGRAPHY, 1977, 130 (JAN11) :287-291
[6]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF 34 SELECTED FLAVONOIDS [J].
DAIGLE, DJ ;
CONKERTON, EJ .
JOURNAL OF CHROMATOGRAPHY, 1982, 240 (01) :202-205
[7]   QUERCETIN AND RUTIN AS INHIBITORS OF AZOXYMETHANOL-INDUCED COLONIC NEOPLASIA [J].
DESCHNER, EE ;
RUPERTO, J ;
WONG, G ;
NEWMARK, HL .
CARCINOGENESIS, 1991, 12 (07) :1193-1196
[8]   DIHYDROCHALCONE SWEETENERS - SYNTHESIS, SENSORY EVALUATION, AND CHIRAL ELUANT CHROMATOGRAPHY OF THE D-ANTIPODE AND L-ANTIPODE OF A POTENTLY SWEET, SUCROSE-LIKE HOMOSERINE-DIHYDROCHALCONE CONJUGATE [J].
DUBOIS, GE ;
STEPHENSON, RA .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1982, 30 (04) :676-681
[9]   HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC METHOD FOR RESOLUTION AND QUANTITATION OF NARINGIN AND NARINGENIN RUTINOSIDE IN GRAPEFRUIT JUICE [J].
FISHER, JF ;
WHEATON, TA .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1976, 24 (04) :898-899
[10]   EFFECT OF NEODIOSMIN ON THRESHOLD AND BITTERNESS OF LIMONIN IN WATER AND ORANGE JUICE [J].
GUADAGNI, DG ;
MAIER, VP ;
TURNBAUGH, JG .
JOURNAL OF FOOD SCIENCE, 1976, 41 (03) :681-684