THE SCANNING FORCE MICROSCOPY OF DNA IN AIR AND IN N-PROPANOL USING NEW SPREADING AGENTS

被引：42

作者：

SCHAPER, A ^{[1
]}

STARINK, JPP ^{[1
]}

JOVIN, TM ^{[1
]}

机构：

[1] MAX PLANCK INST BIOPHYS CHEM,DEPT MOLEC BIOL,D-37018 GOTTINGEN,GERMANY

来源：

FEBS LETTERS | 1994年 / 355卷 / 01期

关键词：

ATOMIC FORCE MICROSCOPY; SCANNING TUNNELING MICROSCOPY; DNA; CETYLPYRIDINIUM CHLORIDE; BENZYLALKONIUM CHLORIDE; 2,4,6-TRIS(DIMETHYLAMINOMETHYL)PHENOL;

D O I：

10.1016/0014-5793(94)01166-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present recent advances in DNA specimen preparation technique for scanning force microscopy (SFM) based on spreading on mica in the presence of cationic and non-ionic detergents. Reproducible DNA imaging in air and in n-propanol has been achieved in the presence of the non-ionic detergent 2,4,6-tris(dimethylaminomethyl) phenol (DMP-30) or the cationic detergent cetylpyridinium chloride (CP) in a microdrop containing nanograms of DNA. In an alternative procedure, a microdrop of detergent is applied to the surface just prior to the DNA. Quantitative image analysis yields as the apparent molecular dimensions of the DNA a width of similar to 7 nm and a height of similar to 0.7 nm, and delineates the problems of DNA metrology by SFM.

引用

页码：91 / 95

页数：5

共 28 条

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